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2 edition of Long-term organ culture of amphibian liver. found in the catalog.

Long-term organ culture of amphibian liver.

Norman Fleming

Long-term organ culture of amphibian liver.

by Norman Fleming

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  • 2 Currently reading

Published by University of East Anglia in Norwich .
Written in English


Edition Notes

Thesis (Ph.D.) -University of East Anglia, School of Biological Sciences, 1974.

ID Numbers
Open LibraryOL13845501M

Long-term cultures of fibroblasts seem to undergo greater variations in enzyme content than, of example, liver cells. Westfall et al. () found that the arginase and rhodanese activities in liver cell lines were high, as in the tissue of origin, but that fibroblast lines varied widely in the activities of one or both of these enzymes. Short-term (1–3 months) and long-term (6–8 months) reconstitution obtained after the transfer of – dpc YS-derived (A) and Sp-derived (B) precursors after 4 days in organ culture and of 10– dpc AGM (C) into Rag2−/− (in gray) or Rag2γc−/− (in white) recipient mice. Long-term reconstitution was considered to be present.

Organ culture system. The need for constant supply of nutrients renders ex vivo machine perfusion the only feasible approach for organ culture of all highly vascular organs that require vascular anastomosis to the recipient, thus organ culture and machine perfusion will be used interchangeably. Nutrient- and oxygen-rich perfusate can be actively pumped into the tissue through an existing. Direct induction of vitellogenin production in cultured male amphibian hepatocytes by estradiol beta has been accomplished. Liver cells were isolated from adult male bullfrogs by collagenase.

A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture, Lab Chip, , /c3 — RSC. The Liver:Facts, Functions, and Structure of The liver is the largest organ in the entire, normal human body. It weighs anywhere from to pounds. With its large size it is also a very resilient organ. Up to 3/4 of its cells can be removed before is ceases to function. It is red-brown organ roughly shaped like a .


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Long-term organ culture of amphibian liver by Norman Fleming Download PDF EPUB FB2

Long-term amphibian organ culture. Brown D, Fleming N. Methods in Cell Biology, 01 JanDOI: Hepatocyte function in long-term organ culture of Amphimuma means liver.

Fleming N, Brown D, Balls M. J Cell Sci, 18(3), 01 Aug Cited by: 1. Since a variety of tissues from adults of a number of amphibian species survive and function normally in long-term organ culture, it is argued that such cultures have great potential value for.

GENERAL AND COMPARATIVE ENDOCRINOL () Amphibian Pancreas Function in Long-Term Organ Culture Control of Insulin Release STEVEN GATER AND MICHAEL BALLS Department of Human Morphology, University of Nottingham Medical School, Nottingham NG7 2UH, England Accepted Pancreas organ cultures from the adult urodele amphibian Cited by: HEPATOCYTE FUNCTION IN LONG-TERM ORGAN CULTURE OF AMPHIUMA MEANS LIVER N.

FLEMING,* D. BROWN AN MD. BALLSf School of Biological Sciences, University of East Anglia, Norwich NR4 7TJ, England SUMMARY Fragments of liver from the adult urodel Amphiumae means, the Congo eel, were maintained in organ cultur foer up to 70 by:   LONG-TERM AMPHIBIAN ORGAN CULTURE „ 6-S ', i i i * i 1 " 1 " i 1 + ••.

^ 2- 1 J 1- 1) 7 1 14 1 21 1 28 1 35 1 42 1 49 1 1 1 56 63 70 Days in culture Fig. Glycogen content of A. means liver fragments over a week culture period (each Cited by: Organ cultures have been used to study mammalian morphogenesis; adult mammalian lung, prostate, skin and intestine survive well, while liver, kidney, pancrea and splees dno not.

In earlier experiments, we found that adult amphibian tissues from several species survived wel iln long-term organ culture Th. e effects of culture on mitotic incidence.

Liver fragments survived better in media based on MEM or BME than in medium based on Leibovitz L Since many aspects of tissue-specific structure and function are retained, long-term amphibian organ culture is well suited to studies on the control of hepatocyte function and on the effects of metabolites, hormones, drugs and toxins.

Fragments of pancreas, liver, spleen, kidney and lung from Amphiuma means, the Congo eel, were maintained in organ culture for up to 35 days in a modified Leibovitz L15 medium.

These Amphiuma tissues retained their typical histological features throughout the culture period, surviving better and for longer than tissues from other amphibian species. 3. The success of long-term organ cultures of amphibian tissues may be due to their low respiration rates and large cell sizes.

INTRODUCTION IN GENERAL, adult amphibian tissues survive better in long-term organ culture than adult mammalian tissues, but there are considerable variations between different amphibian species in this respect. It is concluded that long-term amphibian organ culture will be a useful technique for the pharmacologist.

Acknowledgement-D. Brown was supported by the Science Research Council. REFERENCES BAILEY N. () Statistical Methods in Biology. English Universities Press, London.

BALLS M. BROWN D. & FLEMING N. () Long-term amphibian organ. In in vitro cultures of liver from Ambystoma mexicanum glycogenolysis was stimulated by adrenaline, glucagon, and vasopressin in a dose-dependent manner. Maximum activity was seen at 10 −6 M hormone while 10 −9 M was without effect.

Dibutyryl cyclic AMP (10 −3 M) stimulated glycogenolysis maximally although 10 −5 M had no effect. The glucose release brought about by. Long-term organ culture of amphibian liver.

Author: Fleming, N. ISNI: Awarding Body: University of East Anglia Current Institution: University of East Anglia Date of Award: Availability of Full Text: Full text unavailable from EThOS.

Two distinct types of cells were derived from organ cultures of liver from adult and larval Xenopus laevis. Each type was isolated in clonal cell culture. Several media were compared with respect to support of epithelioid outgrowths from explants and support of growth of epithelioid colonies in cell culture.

Ultracentrifuged embryo extract promotes the growth of all cell types, but the. Amphibian pancreas function in long-term organ culture: control of amylase release. Gater S, Balls M Gen Comp Endocrinol, 33(1), 01 Sep Since liver is the main target organ for accumulation and detoxification of xenobiotics [29], it is highly probable that frogs lived in areas with intensive herbicides usage would show enlarge.

Introduction. The liver is mainly composed of two epithelial cell types, hepatocytes and ductal cells. Hepatocytes synthesize essential serum proteins, control metabolism, and detoxify a wide variety of endogenous and exogenous molecules (Duncan et al., ).Despite their considerable replication capacity in vivo (Michalopoulos, ), hepatocytes have resisted long-term expansion in culture.

The structure and ultrastructure of the renal tubule of the urodele amphibian, Amphiuma means Article (PDF Available) in Journal of Anatomy (Pt 3) January with 79 Reads. Fragments of pancreas from the adult urodele amphibian Amphiuma means (the Congo eel) were maintained in organ culture for up to 28 days.

Both amylase synthesis and release into the culture. Methods Cell Biol. ; Long-term amphibian organ culture. Brown D, Fleming N. PMID: [PubMed - indexed for MEDLINE] Publication Types.

The technique of in vitro culture which involves the maintenance or growth of organs is called organ culture. In this technique the structure and function of the organ is preserved in such a way that it closely resembles the same organ in organs are difficult to study under experimental conditions, because they are not well suited to experimental manipulation and are inaccessible.

The relationship between cell sizes, respiration rates and survival of amphibian tissues in long-term organ cultures. Comp Biochem Physiol A Comp Physiol.

Mar 1; 44 (3)– Monnickendam MA, Balls M. Organ culture of adult Amphiuma means kidney: effects of media and urea on ammonia production, glycogen content and cell proliferation.In the developing mouse embryo the first definitive (transplantable-into-the-adult) haematopoietic stem cells/long-term repopulating units (HSC/RUs) emerge in the AGM region and umbilical vessels on days post coitum (d.p.c.).

Here, by limiting dilution analysis, we anatomically map the development of definitive HSC/RUs in different embryonic tissues during early colonisation of the liver.The requirements for establishment and survival of primary cultures of larval amphibian liver cells were investigated.

Plating efficiency was found to be enhanced by a collagen substrate, by diluted conditioned medium from an adultXenopus kidney cell line and by high initial cell densities. Plating efficiency was highest at a tonicity of – mOsm/kg.